The partnership, unveiled at CES 2019 following successful trials, will see Mobileye-fitted vehicles gather large volumes of location data on road networks and roadside infrastructure, including lamp posts, manhole covers, and road markings.
This information will then be cross-referenced with existing geospatial datasets, such as OS MasterMap, to help develop accurate maps of Britain's roads and surrounding infrastructure.
Utility companies, for example, can use the service to maintain the precise location of their assets on the ground. By enabling a stronger view into overground and underground assets, these companies can more efficiently plan and manage maintenance needs, support or other necessary work.
The new data is also designed to support the successful rollout of technologies for new market segments, including 5G, intelligent mobility and connected autonomous vehicles.
Ordnance Survey chief executive Neil Ackroyd said: "At OS we work hard to enable an environment that supports new technologies and data services across the public and private sector. OS appreciates its role as a trusted advisor in existing and new markets and will continue to invest and innovate to support our utility customers and in new discovery projects such as CAV, 5G and IoT.
"One key, and common, learning is that detailed and accurate geospatial data is a must for the success of these projects. We envisage this new rich data to be key to how vehicles, infrastructure, people and more will communicate in the digital age.
"Our partnership with Mobileye further enhances our commitment to supporting Britain as a world-leading centre for digital and tech excellence."
Mobileye president and CEO Professor Amnon Shashua added: "Using maps to improve operations between businesses and cities will bring us closer to the realisation of smart cities and safer roads."